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| Status |
Public on Oct 03, 2022 |
| Title |
CGS15-2 |
| Sample type |
SRA |
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| Source name |
Bacteria
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| Organism |
Corynebacterium glutamicum ATCC 13032 |
| Characteristics |
strain: CGS15
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA of each sample was extracted using the RNeasy Mini Kit (Qiagen, Beijing, China) using cells grown to the mid-exponential phase at 30 ℃ and 220 rpm.
RNA libraries were prepared for sequencing using standard Illumina protocols
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Data processing |
Prokaryote mRNA sequencing by Illumina HiSeq
Sequencing was carried out using a 2x150 paired-end (PE) configuration
image analysis and base calling were conducted by the HiSeq Control Software (HCS) + OLB + GAPipeline-1.6 (Illumina) on the HiSeq instrument
technical sequences were removed by Cutadapt (version 1.9.1) and clean data were aligned to reference genome via software Bowtie2 (v2.2.6).
Genome_build: GCA_000011325.1
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| Submission date |
Sep 19, 2021 |
| Last update date |
Oct 03, 2022 |
| Contact name |
Xi Sun |
| E-mail(s) |
sunxi@tju.edu.cn
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| Organization name |
Tianjin university
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| Street address |
Yaguan road 135
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| City |
Tianjin |
| ZIP/Postal code |
300072 |
| Country |
China |
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| Platform ID |
GPL30649 |
| Series (1) |
| GSE184402 |
Global cellular metabolic rewiring adapts Corynebacterium glutamicum to efficient non-natural substrate utilization |
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| Relations |
| BioSample |
SAMN21507113 |
| SRA |
SRX12254445 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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