|
| Status |
Public on Sep 27, 2021 |
| Title |
O8_Veh |
| Sample type |
SRA |
| |
|
| Source name |
MSC
|
| Organism |
Mus musculus |
| Characteristics |
tissue: mouse bone marrow
cell type: MSCs
age: old
|
| Treatment protocol |
MSCs was 1 µM melatonin treated for 24 hours.
|
| Growth protocol |
MSCs was cultured in DMEM medium with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using BGISEQ500 protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Data processing |
Basecalls performed using BGISEQ-500
SOAPnuke was used to get the "clean reads".
Mapping clean reads to Mus_musculus whole genome using BWA.
Bowtie2 was used to get the mapping ratio.
Fragments Per Kilobase Million (FPKM) was calculated based on the RSEM.
Genome_build: Mus musculus (mm39)
Supplementary_files_format_and_content: fpkm, counts, tpm values for each Sample
|
| |
|
| Submission date |
Sep 23, 2021 |
| Last update date |
Sep 27, 2021 |
| Contact name |
Liu Zhiqiang |
| E-mail(s) |
zhiqiangliu@tmu.edu.cn
|
| Organization name |
Tianjin Medical University
|
| Street address |
22 Qxiangtai Rd
|
| City |
Tianjin |
| ZIP/Postal code |
300070 |
| Country |
China |
| |
|
| Platform ID |
GPL23479 |
| Series (2) |
| GSE184712 |
Melatonin Enhances Osteoblastogenesis from Senescent Mesenchymal Stem Cells via NSD2 Mediated Chromatin Remodeling [RNA-seq] |
| GSE184714 |
Melatonin Enhances Osteoblastogenesis from Senescent Mesenchymal Stem Cells via NSD2 Mediated Chromatin Remodeling. |
|
| Relations |
| BioSample |
SAMN21584146 |
| SRA |
SRX12325119 |
