|
| Status |
Public on Jun 30, 2010 |
| Title |
Liver from the rats treated with 1.5 mg/kg animal of dexamethasone and 0.7 g/kg animal of AICAR for 8 hours, rep 1. |
| Sample type |
RNA |
| |
|
| Source name |
Rat liver, excised 8 hours
|
| Organism |
Rattus norvegicus |
| Characteristics |
agent: dexamethasone + AICAR
gender: male
strain: Sprague-Dawley
weight: 180-200g
|
| Treatment protocol |
The animal study was approved by the NICHD Animal Care and Use Committee (protocol numbers: ASP07-016 and ASP09-030). Male Sprague-Dawley rats (180-200g) were injected with either AICAR (0.7 g/kg animal) or physiologic saline into their peritoneal cavities. Animals in each group were also injected intramuscularly with dexamethasone (1.5 mg/kg) or normal saline. Eight hours after the injection, animals were euthanized using CO2, and their livers were harvested for total RNA purification.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by using RNAeasy mini kit (Qiagen, Valencia, CA)
|
| Label |
biotin
|
| Label protocol |
cRNA was produced and labeled with the One-Cycle Target Labeling and Control Reagents Kit (Affymetrix, Inc, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
We employed a standard hybridization method indicated by Affimetrix.
|
| Scan protocol |
We used the GeneChip Fluidics Station 450 (Affimetrix) and the GeneChip Scanner 3000 7G System for washing and scanning the micorarrays.
|
| Description |
None
|
| Data processing |
The raw data (.cel files) were processed using Bioconductor Affy R package. The normalization method is RMA.
|
| |
|
| Submission date |
Jun 30, 2010 |
| Last update date |
Jun 30, 2010 |
| Contact name |
Tomoshige Kino |
| E-mail(s) |
kinot@mail.nih.gov
|
| Phone |
(301)496-6417
|
| Fax |
(301) 402-0884
|
| Organization name |
National Institute of Child Health & Human Development
|
| Department |
Program in Reproductive and Adult Endocrinology
|
| Lab |
Section on Pediatric Endocrinology
|
| Street address |
10 Center Drive MSC 1109
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE22647 |
Adenosine 5’ monophosphate-activated protein kinase regulates metabolic actions of glucocorticoids by phosphorylating the glucocorticoid receptor through p38 mitogen-activated protein kinase. |
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