|
| Status |
Public on Apr 08, 2011 |
| Title |
LCM_primary_lung_tumor_stroma549 |
| Sample type |
RNA |
| |
|
| Source name |
primary lung cancer
|
| Organism |
Homo sapiens |
| Characteristics |
age: 74
Sex: F
disease state: primary lung cancer
histology: Adenocarcinoma
Stage: III
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Stromal tissue was isolated by Laser Capture Microdissection (LCM) using the CellCut Plus LCM (Molecular Machines & Industries, Glattbrugg, Switzerland) mounted on Olympus IX81 inverted microscope. Subsequently, RNA was isolated using Total RNA purification kit (Norgen Biotek, Canada) and RNA was amplified using RNA amplification kit (WT-OvationTM Pico RNA Amplification System Version 1.0, NuGEN, San Carlos, CA).
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix procedures
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Whole-transcript expression profiling
NSCLC_stroma549
|
| Data processing |
Raw data was pre-processed and normalized using RMA implemented in Affymetrix Expression console v1.1, expression values were summarized at the gene-core level based on NetAffx NA28 annotation library integrated in Affymetrix Expression console v1.1.
|
| |
|
| Submission date |
Jul 09, 2010 |
| Last update date |
Aug 20, 2013 |
| Contact name |
Ming-Sound Tsao |
| E-mail(s) |
Ming.Tsao@uhn.on.ca
|
| Phone |
(416) 946-4426
|
| Organization name |
Ontario Cancer Institute/ Princess Margaret Hospital
|
| Street address |
|
| City |
Toronto |
| ZIP/Postal code |
M5G 2M9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL5175 |
| Series (2) |
| GSE22863 |
Prognostic Gene Expression Signature of Carcinoma Associated Fibroblasts in Non-Small Cell Lung Cancer [expression profiling_NSCLC stroma] |
| GSE22874 |
Prognostic Gene Expression Signature of Carcinoma Associated Fibroblasts in Non-Small Cell Lung Cancer |
|
