|
| Status |
Public on Jul 13, 2010 |
| Title |
CD4Tcell-Th1-restimulated12hour-2 [HG-U133B] |
| Sample type |
RNA |
| |
|
| Source name |
CD4+ T cells from PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral blood
cell type: CD4+ T cells
treatment agent: aCD3/aCD28
time post-treatment: 12 hrs
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
cRNA was hybridized on GeneChip HGU133B.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from Th1-polarized cells 12 hrs post-restimulation
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Jul 13, 2010 |
| Last update date |
Jul 13, 2010 |
| Contact name |
Alex Abbas |
| E-mail(s) |
abbas@gene.com
|
| Organization name |
Genentech, Inc.
|
| Department |
Bioinformatics
|
| Street address |
1 DNA Way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL97 |
| Series (1) |
| GSE22886 |
Expression profiles from a variety of resting and activated human immune cells |
|
