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Sample GSM5678111 Query DataSets for GSM5678111
Status Public on Apr 15, 2022
Title T00275909
Sample type RNA
 
Source name kidney, Hydralazine 10 milligram per kilogram per day
Organism Rattus norvegicus
Characteristics tissue: kidney
Sex: male
age: 16 weeks
strain: WKY/Izm
individual: W311-25 W311-26
scandate: 2005-07-02
compound: Hydralazine
dose: 10
Extracted molecule total RNA
Extraction protocol Tissues were obtained from male rats at 16 weeks old. Total RNA was extracted using RNeasy maxi kit (Qiagen).
Label Cy5
Label protocol All reagents were provided in the CodeLink Expression Assay Kit. cRNA synthesis was performed according to the manufacturer's instructions using 2 ug of total RNA and labeled with Cy5.
 
Hybridization protocol The fragmented cRNA was hybridized overnight at 37C in hybridization buffer in an Innova 4080 shaking incubator (New Brunswick, Edison, NJ) at 300 rpm.
Scan protocol Images were captured on an Axon GenePix Scanner (Arlington, TX) using GenePix Pro 6.0 Software and were analyzed using CodeLink Expression Analysis Software.
Data processing Expression values were globally normalized to the median expression value of the whole rat probes. Signal smaller than or equal to 0.23 is weak and such probes should be discarded.
 
Submission date Nov 06, 2021
Last update date Apr 15, 2022
Contact name Fumihiko Takeuchi
E-mail(s) fumihiko@takeuchi.name
Organization name National Center for Global Health and Medicine
Department Research Institute
Street address 1-21-1 Toyama
City Shinjuku-ku
State/province Tokyo
ZIP/Postal code 162-8655
Country Japan
 
Platform ID GPL2896
Series (2)
GSE188349 Transcriptomic response in the heart and kidney to different types of antihypertensive drug administration [kidney]
GSE188350 Transcriptomic response in the heart and kidney to different types of antihypertensive drug administration

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
67071 24.357
296048 0.99
301004 0.187
206040 10.568
43019 1.335
188041 18.362
191021 0.794
81071 0.349
293017 18.128
78010 5.575
97103 2.525
151074 1.063
158040 1.369
92111 0.238
274088 0.272
302097 0.08
198072 0.753
243092 1.586
29106 0.657
234078 6.246

Total number of rows: 33849

Table truncated, full table size 419 Kbytes.




Supplementary file Size Download File type/resource
GSM5678111_T00275909.txt.gz 181.2 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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