|
Status |
Public on Dec 20, 2021 |
Title |
day 10 anti PD-1 + muGITR-L bispecific mouse 3 Draining lymph node |
Sample type |
RNA |
|
|
Source name |
draining lymph node
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6J tissue: draining lymph nodes treatment: anti-PD-1 + muGITR-L bispecific time point: day 10
|
Treatment protocol |
FFPE blocks prepared.
|
Growth protocol |
The adherent colorectal carcinoma cell line CT26 (cat. # CRL-2638, ATCC, Manassas, VA) and mammary gland adenocarcinoma cell line JC (cat. # CRL-2116, ATCC, Manassas, VA) were expanded and maintained in RPMI-1640 (Roswell Park Memorial Institute medium cat. # SH30021.02 GE Healthcare Life Sciences) supplemented with 10% FBS (cat. # F442-500 Sigma-Aldrich).
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA processed from formalin fixed paraffin embedded (FFPE) blocks as per NanoStringTM protocols.
|
Label |
n/a
|
Label protocol |
Labeled as to group, mouse number and tissue.
|
|
|
Hybridization protocol |
Samples processed as per NanoStringTM protocols.
|
Scan protocol |
NanoStringTM nCounter.
|
Description |
group 5 48 DLN
|
Data processing |
Normalization method NanoStringTM nSolver 4.0 advanced analysis, normalised to standards on NanoStringTM panel per their protocol.
|
|
|
Submission date |
Nov 22, 2021 |
Last update date |
Dec 20, 2021 |
Contact name |
Kyle Halliwill |
E-mail(s) |
kyle.halliwill@gmail.com
|
Organization name |
Abbvie Inc
|
Street address |
1500 Seaport Blvd
|
City |
Redwood City |
State/province |
California |
ZIP/Postal code |
94063 |
Country |
USA |
|
|
Platform ID |
GPL30298 |
Series (1) |
GSE189359 |
Gene expression based outcome of CT-26 tumor in C57/BL6 mice after treatment with anti-PD-1 antibody, GITR-L, combination or Bispecific molecules |
|