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Sample GSM5699845 Query DataSets for GSM5699845
Status Public on Dec 20, 2021
Title day 8 anti PD-1 mouse 3 tumor
Sample type RNA
 
Source name tumor
Organism Mus musculus
Characteristics strain: C57BL/6J
tissue: CT26 tumor
treatment: anti-PD-1
time point: day 8
Treatment protocol FFPE blocks prepared.
Growth protocol The adherent colorectal carcinoma cell line CT26 (cat. # CRL-2638, ATCC, Manassas, VA) and mammary gland adenocarcinoma cell line JC (cat. # CRL-2116, ATCC, Manassas, VA) were expanded and maintained in RPMI-1640 (Roswell Park Memorial Institute medium cat. # SH30021.02 GE Healthcare Life Sciences) supplemented with 10% FBS (cat. # F442-500 Sigma-Aldrich).
Extracted molecule total RNA
Extraction protocol Total RNA processed from formalin fixed paraffin embedded (FFPE) blocks as per NanoStringTM protocols.
Label n/a
Label protocol Labeled as to group, mouse number and tissue.
 
Hybridization protocol Samples processed as per NanoStringTM protocols.
Scan protocol NanoStringTM nCounter.
Description group 2 8 tumor
Data processing Normalization method NanoStringTM nSolver 4.0 advanced analysis, normalised to standards on NanoStringTM panel per their protocol.
 
Submission date Nov 22, 2021
Last update date Dec 20, 2021
Contact name Kyle Halliwill
E-mail(s) kyle.halliwill@gmail.com
Organization name Abbvie Inc
Street address 1500 Seaport Blvd
City Redwood City
State/province California
ZIP/Postal code 94063
Country USA
 
Platform ID GPL30298
Series (1)
GSE189359 Gene expression based outcome of CT-26 tumor in C57/BL6 mice after treatment with anti-PD-1 antibody, GITR-L, combination or Bispecific molecules

Supplementary data files not provided
Processed data are available on Series record

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