|
| Status |
Public on Aug 01, 2011 |
| Title |
Adipose_AfricanAmerican_4648891029_C |
| Sample type |
RNA |
| |
|
| Source name |
Subcutaneous Adipose tissue
|
| Organism |
Homo sapiens |
| Characteristics |
disease: obese
age range: 18-25 yr old
ethnicity: African American
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
4648891029_C.AVG_Signal
|
| Data processing |
The data were normalised using quantile normalisation with Genome studio software from Illumina
|
| |
|
| Submission date |
Aug 09, 2010 |
| Last update date |
Aug 01, 2011 |
| Contact name |
Swapna Mahurkar |
| E-mail(s) |
mahurka@usc.edu
|
| Phone |
3234423198
|
| Organization name |
University of Southern California
|
| Department |
Preventive Medicine, Keck School of Medicine
|
| Lab |
Childhood obesity research center
|
| Street address |
2250 Alcazar St
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE23506 |
Differences in subcutaneous adipose tissue gene expression between obese African Americans and Hispanic Youths |
|
