|
| Status |
Public on Jan 12, 2022 |
| Title |
mTSC with induced Hand1 expression |
| Sample type |
SRA |
| |
|
| Source name |
mouse TSC
|
| Organism |
Mus musculus |
| Characteristics |
time point: D2
cell type: mouse TSC
treatment: 0.6 ug/ml dox
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mouse TSC differentiating for 2 days without or with induced Hand1 expression by doxycycline treatment.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Data processing |
Zebracall software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using Tophat with default parameters
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using EdgeR package of R.
Genome_build: mm10
|
| |
|
| Submission date |
Jan 07, 2022 |
| Last update date |
Jan 12, 2022 |
| Contact name |
Jinhua Lu |
| E-mail(s) |
jinhua888@126.com
|
| Organization name |
Xiamen University
|
| Street address |
No. 4221, Xiangan Road, Xiangan District, Xiamen city
|
| City |
Xiamen |
| ZIP/Postal code |
361000 |
| Country |
China |
| |
|
| Platform ID |
GPL23479 |
| Series (1) |
| GSE193214 |
Adgrg1 is a New Transcriptional Target of Hand1 During Trophoblast Giant Cell Differentiation |
|
| Relations |
| BioSample |
SAMN24720736 |
| SRA |
SRX13653999 |
