|
| Status |
Public on May 31, 2022 |
| Title |
RAW38C3 |
| Sample type |
SRA |
| |
|
| Source name |
Raw264.7.
|
| Organism |
Mus musculus |
| Characteristics |
treatment: 38°C
|
| Treatment protocol |
For temperature experiments, cells were seeded and allowed to adhere at 37°C. The cells were subsequently shifted to incubators set to 34°C, 37°C, or 38°C
|
| Growth protocol |
Raw264.7. cells were grown in DMEM supplemented with 10% fetal bovine serum and 1% Penicillin/Streptomycin (Invitrogen).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After 12h RNA was extracted using RNATri (Bio&Sell) followed by DNaseI digestion.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
For alignment and quantification of gene.tpm files we used the Whippet-quant function of Whippet v0.11. This aligns and quantifies in one step
|
| |
|
| Submission date |
Jan 13, 2022 |
| Last update date |
May 17, 2023 |
| Contact name |
Marco Preussner |
| E-mail(s) |
mpreussner@zedat.fu-berlin.de
|
| Organization name |
FU berlin
|
| Lab |
Heyd
|
| Street address |
Takustrasse 6
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL21103 |
| Series (1) |
| GSE193639 |
Body temperature variation controls pre-mRNA processing and transcription of anti-viral genes |
|
| Relations |
| BioSample |
SAMN24962016 |
| SRA |
SRX13778818 |
