|
| Status |
Public on Nov 24, 2010 |
| Title |
8688T3 tumor treated, grade3 |
| Sample type |
RNA |
| |
|
| Source name |
8688T3 tumor treated, grade3
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/B6-129SvJae
tissue: lung tumor
gender: female
|
| Treatment protocol |
For treated samples tamoxifen was administered IP in 2 injections. Tumors were collected 7 days after first injection.
|
| Growth protocol |
Tumors were extrracted from 10 week old animals
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared from 100 ng total RNA using Nugen Ovation RNA amplification system V2 according to the manufacturers instructions.
|
| |
|
| Hybridization protocol |
Following fragmentation, 4 ug of cDNA was hybridized for 16 hr at 45C on Mouse 430A_2 chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the affymetrix 3000 7G scanner.
|
| Description |
Gene expression data from microdissected p53 restored adenocarcinomas
|
| Data processing |
R version 2.11.0 (x86_64-pc-linux-gnu), affyPLM_1.24.0, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.0, Biobase_2.8.0
|
| |
|
| Submission date |
Aug 30, 2010 |
| Last update date |
Nov 24, 2010 |
| Contact name |
Charles Arthur Whittaker |
| E-mail(s) |
charliew@mit.edu
|
| Organization name |
Koch Institute
|
| Street address |
77 Mass Ave 76-189
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02152 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (2) |
| GSE23874 |
Stage-specific sensitivity to p53 restoration in lung cancer: tumor data |
| GSE23875 |
Stage-specific sensitivity to p53 restoration in lung cancer |
|
