|
| Status |
Public on Jul 21, 2011 |
| Title |
MMTV-PymT 5 |
| Sample type |
RNA |
| |
|
| Source name |
female mouse primary mammary tumor
|
| Organism |
Mus musculus |
| Characteristics |
genetically engineered mouse model: MMTV-PymT
tissue type: mammary tumor
strain: FVB
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from tumor and normal tissues using the mirVana miRNA Isolation kit (Ambion) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA from 1 ug total RNA were prepared using Affymetrix One Cycle Target Labeling kit.
|
| |
|
| Hybridization protocol |
cRNA were hybridized onto GeneChip Mouse Genome 430A 2.0 Array.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip scanner 3000.
|
| Description |
Gene expression data of mouse primary mammary tumors
|
| Data processing |
Data were collected using Affymetrix GCOS software and then normalized using GC-RMA of Partek Genomic Suite (www.partek.com). The normalized data was further filtered using MAS5 detection calls for probes designated as “P” (present) or “M” (Marginal) in less than 3 samples of all data population.
|
| |
|
| Submission date |
Sep 01, 2010 |
| Last update date |
Jul 21, 2011 |
| Contact name |
Jeffrey E Green |
| E-mail(s) |
JEGreen@mail.nih.gov
|
| Phone |
3014355193
|
| Fax |
3014968709
|
| Organization name |
NCI
|
| Street address |
Rm 4054, 37 Convent Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE23938 |
Comprehensive genomic profiling identified miRNA signatures associated with mammary tumor differentiation and development |
|
