|
| Status |
Public on Aug 17, 2011 |
| Title |
MEF Nelf-b KO rep1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse embryonic fibroblast derived from a Nelf-bfl/- embryo
|
| Organism |
Mus musculus |
| Characteristics |
cell type: embryonic fibroblasts
treatment: MEF infected with Cre-expressing virus
strain: mixed background of 129/SvJ, C57BL/6, and FVB
|
| Treatment protocol |
MEFs were infected with Cre-expressing retrovirus and selected with 2mg/ml puromycin for 7 days.
|
| Growth protocol |
MEFs were maintained in Dulbecco's modification of Eagle's medium supplemented with 10% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted form the virus-infected cells with miRNeasy mini kit (Qiagen) according to manufacturer's instruction.
|
| Label |
Cy3
|
| Label protocol |
RNAs were labeled using the Illumina TotalPrep RNA Amplification Kit (Ambion, #AMIL1791).
|
| |
|
| Hybridization protocol |
Standard Ilumina procotol as described in previous publication (Genome Research 14: 2347).
|
| Scan protocol |
BeadChips were scanned on a BeadArray Reader (illumina) using BeadScan software (v3.2, Illumina).
|
| Description |
replicate 1
|
| Data processing |
Scanned data were uploaded into GenomeStudio software ( v1.1, Illumina) via the gene expression module (Direct Hyb).
|
| |
|
| Submission date |
Sep 13, 2010 |
| Last update date |
Aug 17, 2011 |
| Contact name |
Jianlong Sun |
| E-mail(s) |
jianlongs@gmail.com
|
| Phone |
210-273-4879
|
| Organization name |
Boston Children's Hospital
|
| Department |
Hematology/Oncology
|
| Lab |
Fernando Camargo
|
| Street address |
1 Blackfan Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE24114 |
Regulation of Mammalian Cell Growth and Survival By an RNA Polymerase II-Pausing Factor (Gene expression) |
| GSE24180 |
Regulation of Mammalian Cell Growth and Survival By an RNA Polymerase II-Pausing Factor |
|
