|
| Status |
Public on May 23, 2011 |
| Title |
STZ + insulin 5 |
| Sample type |
RNA |
| |
|
| Source name |
Retina
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
treatment: STZ + insulin
tissue: retina
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep kit for Illumina arrays. Cy3-Streptavidin was incorporated during Wash and Block protocol as a fluor for scanning.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Replicate 5
|
| Data processing |
The data were normalized using average normalization in GenomeStudio v1.0.6
|
| |
|
| Submission date |
Sep 28, 2010 |
| Last update date |
May 23, 2011 |
| Contact name |
Penn State COM |
| Organization name |
Penn State College of Medicine
|
| Department |
Genome Sciences Facility
|
| Street address |
500 University Drive
|
| City |
Hershey |
| State/province |
PA |
| ZIP/Postal code |
17033 |
| Country |
USA |
| |
|
| Platform ID |
GPL10239 |
| Series (1) |
| GSE24423 |
Transcriptomic characterization of the diabetic retinal response to chronic insulin treatment |
|
