|
| Status |
Public on Sep 29, 2010 |
| Title |
Patient 15, before month24 IFN-beta injection_chipB |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral blood mononuclear cells
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
age at ifn-beta therapy onset (baseline blood sampling): 42
duration from ms diagnosis to therapy initiation (in months): 1
edss at baseline: 2.0
edss after 1 year: 2.5
edss after 2 years: 3.0
edss after 5 years: 2.5
number of relapses during the year prior to treatment: 1
number of relapses during 1-year follow-up: 1
number of relapses during 2-year follow-up: 1
number of relapses during 5-year follow-up: 5
time from start of therapy to the first relapse (in months): 6
completed years of ifn-beta therapy: >=5
|
| Treatment protocol |
Patients were treated with subcutaneous IFN-beta-1b (Betaferon, Bayer Healthcare) at standard dose (250 µg every other day).
|
| Growth protocol |
Patient blood samples were drawn immediately before first IFN-beta injection as well as two days, one, twelve and twenty-four months post therapy initiation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Blood was separated using isopycnic centrifugation (Ficoll), and peripheral blood mononuclear cells were lysed using chaotropic buffer and cleaned by RNeasy (Qiagen) according to the manufacturers' protocol.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 µg total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 µg of cRNA were hybridized for 16 h at 45°C on Affymetrix HG-U133A and B arrays, respectively. Microarrays were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from a multiple sclerosis patient treated with IFN-beta
|
| Data processing |
The raw probe-level signals were converted to expression values using the MAS5.0 algorithm. Accordingly, probeset specific detection calls (Absent/Present) and changes with respect to baseline (Increase/Decrease/NoChange) were ascertained.
|
| |
|
| Submission date |
Sep 29, 2010 |
| Last update date |
May 31, 2011 |
| Contact name |
Robert Goertsches |
| E-mail(s) |
robert.goertsches@med.uni-rostock.de
|
| Organization name |
University
|
| Department |
Neurology
|
| Lab |
Neuroimmunology
|
| Street address |
Gehlsheimer Strasse 20
|
| City |
Rostock |
| ZIP/Postal code |
18047 |
| Country |
Germany |
| |
|
| Platform ID |
GPL97 |
| Series (1) |
| GSE24427 |
Expression data of multiple sclerosis patients receiving subcutaneous Interferon-beta-1b therapy [U133 A and B] |
|
