|
| Status |
Public on Apr 15, 2022 |
| Title |
Glomerular_29_FSGS |
| Sample type |
RNA |
| |
|
| Source name |
Glomerular RNA from kidney biopsy from patient with focal segmental glomeruloscelrosis
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Glomeruli
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
According to manufacturer's protocols.
|
| Scan protocol |
GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
| Description |
Gene expression data from glomerular compartment of human kidney biopsy
|
| Data processing |
The data were analyzed using RMA method and Human Entrez Gene custom CDF annotation version 19 (Brainarray) on three individual batches. Each of the hybridizations was normalized seperately and expression matrix contains the batch corrected data using Combat.
|
| |
|
| Submission date |
Apr 14, 2022 |
| Last update date |
Apr 15, 2022 |
| Contact name |
Sean Eddy |
| E-mail(s) |
seaneddy@umich.edu
|
| Organization name |
University of Michigan
|
| Street address |
1150 West Medical Center Drive
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL19983 |
| Series (1) |
| GSE200828 |
Microarray profiles from glomerular compartment of kidney biopsies from adults screened for enrollment in the NEPTUNE cohort |
|
