|
| Status |
Public on May 15, 2022 |
| Title |
Rhiarthinge FLAG-ChIP |
| Sample type |
SRA |
| |
|
| Source name |
ovary
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: ovary
genotype: w; pRhino>3xFLAG/V5/Precission/GFP-Rhino(art.hinge)[attP40],rhi[g3m13]/rhi[18-7] ;;
antibody: anti-FLAG antibody (Sigma F1804-1MG)
|
| Growth protocol |
25˚C, 12h dark/light
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Chromatin immunoprecipitation (ChIP) was carried out according to Lee et al. (2006)
ChIP-seq libraries were prepared using the NEB Next Ultra II DNA Library for Illumina (NEB).
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Rhiarthinge_in
Rhino construct with artificial hinge domain
|
| Data processing |
reads were trimmed by removal of the adaptor sequences and to a length of 6-50 bases
reads mapping to the Drosophila melanogaster rRNA precursor and the mitochondrial genome using Bowtie (release 1.3.0) with 0 mismatches were excluded
reads were mapped to the Drosophila melanogaster TE consensus using Bowtie (release 1.3.0) with 3 mismatches for genome wide analysis
Assembly: dm6
Supplementary files format and content: all mapping and unique mapping bigwig files
|
| |
|
| Submission date |
May 08, 2022 |
| Last update date |
May 17, 2022 |
| Contact name |
Lisa Baumgartner |
| E-mail(s) |
lisa.baumgartner@imba.oeaw.ac.at
|
| Organization name |
IMBA
|
| Lab |
Brennecke Lab
|
| Street address |
Dr. Bohr-Gasse 3
|
| City |
Vienna |
| ZIP/Postal code |
1030 |
| Country |
Austria |
| |
|
| Platform ID |
GPL25244 |
| Series (2) |
| GSE202465 |
The Drosophila ZAD zinc finger protein Kipferl guides Rhino to piRNA clusters (ChIP-Seq) |
| GSE202468 |
The Drosophila ZAD zinc finger protein Kipferl guides Rhino to piRNA clusters |
|
| Relations |
| BioSample |
SAMN28155987 |
| SRA |
SRX15206515 |
