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| Status |
Public on Dec 04, 2010 |
| Title |
[E-MTAB-305] skeletal muscle RNA |
| Sample type |
SRA |
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| Source name |
skeletal muscle RNA
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| Organism |
Homo sapiens |
| Characteristics |
material type: organism_part
organismpart: skeletal muscle
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| Biomaterial provider |
Ambion
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| Extracted molecule |
total RNA |
| Extraction protocol |
nucleic_acid_extraction | We purchased total RNA from Ambion (Austin, USA). Each tissue samples was pooled from multiple donors. Librarys were prepared as in Armour CD, Castle JC, Chen R, Babak T, Loerch P, et al. (2009) Digital transcriptome profiling using selective hexamer priming for cDNA synthesis. Nat Methods 6: 647-649.
sequencing | We generated an average of 50 million sequence reads per tissue using an Illumina GA-II sequencer, with sequence lengths of 36 nt (adipose, hypothalamus, liver) and 50 nt (colon, heart, kidney, lung, ovary, skeletal muscle, spleen, testes). We trimmed reads to a common length of 28 nt to avoid aligning sequenced amplification primers.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer II |
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| Description |
Provider: TRON
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| Data processing |
processed data not provided
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| Submission date |
Oct 29, 2010 |
| Last update date |
May 15, 2019 |
| Organization |
European Bioinformatics Institute |
| E-mail(s) |
miamexpress@ebi.ac.uk
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| Lab |
ArrayExpress
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| Street address |
Wellcome Trust Genome Campus
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| City |
Hinxton |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB10 1SD |
| Country |
United Kingdom |
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| Platform ID |
GPL9115 |
| Series (1) |
| GSE25028 |
[E-MTAB-305] ncRNA expression, including snoRNAs, across 11 tissues using polyA-neutral amplification |
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| Relations |
| SRA |
ERX005907 |
| Supplementary data files not provided |
SRA Run Selector |
| Processed data not provided for this record |
| Raw data are available in SRA |
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