|
| Status |
Public on Aug 31, 2011 |
| Title |
Enriched |
| Sample type |
genomic |
| |
|
| Source name |
enriched HMEC-hTERT-p53DD-p110a(H1047R)
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: BEZ235-selected immortal human mammary epithelial cells containing randomly mutagenized PIK3CA(H1047R)
|
| Growth protocol |
HMECs were grown according to standard conditions.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from HMEC cultures using Qiagen DNeasy mini Kit. DNA quality and quantity was assessed using Nanodrop, and agarose gel electrophoresis.
|
| Label |
biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner M10.
|
| Description |
BEZ235-selected immortal human mammary epithelial cells containing randomly mutagenized PIK3CA(H1047R)
|
| Data processing |
CEL files and CHP files (generated by Affymetrix GTYPE using the DM algorithm) were processed by dChip and model-based expression performed using the PM/MM model and copy number calls generated as compared to Affymetrix normal controls.
|
| |
|
| Submission date |
Nov 05, 2010 |
| Last update date |
Aug 31, 2011 |
| Contact name |
Nina Ilic |
| E-mail(s) |
nina_ilic@dfci.harvard.edu
|
| Phone |
617 632 6293
|
| Fax |
617 632 4770
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Cancer Biology
|
| Lab |
Roberts Laboratory-SM912
|
| Street address |
44 Binney Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL3720 |
| Series (2) |
| GSE25170 |
MYC drives resistance to PI3K/mTOR targeted inhibition (Sty SNP array) |
| GSE25173 |
PI3K-targeted therapy can be evaded by gene amplification along the MYC-eukaryotic translation initiation factor 4E (eIF4E) axis |
|
