|
| Status |
Public on May 29, 2022 |
| Title |
3D-1_S1_L001_R1_001 |
| Sample type |
SRA |
| |
|
| Source name |
head tissue
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: head
strain: 5905
condition: day03
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted and purified using RNAiso Plus (TaKaRa, catalog: 9108)
RNA libraries were prepared according to instructions of NEBNext Ultra RNA library Prep Kit for Illumina
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina MiSeq |
| |
|
| Data processing |
For RNA-Seq comparison, Sequencing reads were mapped to human with STAR by default parameter. The read counts for each genes were calculated by HTSeq. The count files were used as input to DESeq for differential expression analysis.
Assembly: dm6
Supplementary files format and content: Counts from HTSeq output were merged into csv table with each gene in rows and each sample in columns.
|
| |
|
| Submission date |
May 24, 2022 |
| Last update date |
May 29, 2022 |
| Contact name |
Ruohong Wang |
| E-mail(s) |
ruohongw3@gmail.com
|
| Phone |
15021841935
|
| Organization name |
Chinese Academy of Sciences
|
| Department |
IRCBC
|
| Street address |
100 Haike Road, 13th building
|
| City |
Shanghai |
| State/province |
Not in US |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platform ID |
GPL16479 |
| Series (1) |
| GSE204740 |
Global Stable-isotope Tracing Metabolomics Reveals System-wide Metabolic Alternations in Aging Drosophila |
|
| Relations |
| BioSample |
SAMN28640689 |
| SRA |
SRX15438399 |
