|
| Status |
Public on Apr 13, 2012 |
| Title |
group B, liver allograft wedge biopsie placebo treatment 3 |
| Sample type |
RNA |
| |
|
| Source name |
liver allograft
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: liver allograft
treatment: liver allografts after 15 min flushing with albumin solution
|
| Treatment protocol |
Group A was administered tacrolimus, 20ng/ml in 1500ml albumin solution ; and Group B was administered only albumin solution. Wedge biopsie of the allograft were harvested after 15 min flushing time.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA (200 ng per sample) was amplified and labeled using the Applause WT-Amp ST and Encore Biotin Module kit (NuGEN, San Carlos, CA)
|
| |
|
| Hybridization protocol |
cDNA were hybridized for 18 hr at 45C on GeneChip Human ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Arrays were scanned on an Affymetrix GeneChip scanner 7G.
|
| Description |
liver tissue in RNAlater, stored at -80°C
Gene expression data from liver allografts after 15 min flushing with albumin solution
|
| Data processing |
Normalized expression signals were calculated from Affymetrix CEL files using RMA (Irizarry et al., 2003).
|
| |
|
| Submission date |
Nov 15, 2010 |
| Last update date |
Apr 13, 2012 |
| Contact name |
Julia Wilflingseder |
| E-mail(s) |
julia.wilflingseder@meduniwien.ac.at
|
| Organization name |
Medical University of Vienna
|
| Department |
Nephrology and Dialysis
|
| Lab |
Laboratory Rainer Oberbauer
|
| Street address |
Währinger Gürtel 18-20
|
| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
| |
|
| Platform ID |
GPL11209 |
| Series (1) |
| GSE25338 |
Prevention of acute liver failure |
|
