|
| Status |
Public on Nov 17, 2010 |
| Title |
OC10_Mock |
| Sample type |
RNA |
| |
|
| Source name |
serous epithelial ovarian cancer cells, mock treated
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: ovary
cell type: serous epithelial ovarian cancer cells
gender: female
treatment: mock
|
| Treatment protocol |
When confluent, cells underwent selective trypsinization to remove fibroblasts and were divided into two dishes and grown until ~70% confluent, at which time they were mock treated or treated with 5 µM 5-azacytidine (5-azaC) for 72 hours. Normal OSE (NO) cells were similarly treated, but due to small numbers of cells, we pooled specimens from five individuals to use for each of the arrays. All specimen cultures were analyzed by immunocytochemical staining using anti-cytokeratin antibodies; only cultures with >90% epithelial content were used for the microarrays.
|
| Growth protocol |
Cells were grown in a 1:1 mixture of Medium 199 and MCDB 105 medium with 15% FBS in a humidifed incubator maintained at 37 degrees Celsius with 5% CO2 until confluent.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA Stat-60.
|
| Label |
biotin
|
| Label protocol |
Affymetrix one cycle IVT.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Description |
OC10-MOCK_8981
|
| Data processing |
RMA normalization with RMAExpress software (http://rmaexpress.bmbolstad.com) using background adjustment, quantile normalization and median polish summarization.
|
| |
|
| Submission date |
Nov 16, 2010 |
| Last update date |
Sep 16, 2011 |
| Contact name |
Susan K. Murphy |
| Organization name |
Duke University Medical Center
|
| Department |
Obstetrics and Gynecology
|
| Street address |
B226 LSRC B Wing, Research Drive, Box 91012
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE25427 |
Gene expression profiles of primary cultured ovarian cells in the presence and absence of a DNA methyltransferase inhibitor |
| GSE25429 |
Gene expression profiles of primary cultured ovarian cells and ovarian cancer cell lines in the presence and absence of a DNA methyltransferase inhibitor |
|
