|
| Status |
Public on Nov 17, 2010 |
| Title |
TOV-21G-Decitabine treated |
| Sample type |
RNA |
| |
|
| Source name |
ovarian cancer cells treated with 5 µM decitabine for 72 hours
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: TOV-21G
cell type: ovarian cancer cells
gender: female
treatment: decitabine
|
| Treatment protocol |
At ~70% confluence, cells were either mock treated with tissue culture media (Mock) or with 5 µM 5-aza-2'-deoxxycytidine (Decitabine) for 72 hours.
|
| Growth protocol |
All cells, including sorted CD133+ and CD133- A2780 and PEO1 cells, were grown in RPMI1640 media with 10% FBS in a humidifed incubator maintained at 37 degrees Celsius with 5% CO2 until ~70% confluent.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA Stat-60.
|
| Label |
biotin
|
| Label protocol |
Affymetrix one cycle IVT.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Description |
9938_TOV-21G-D
Experiment 1.
|
| Data processing |
RMA normalization with RMAExpress software (http://rmaexpress.bmbolstad.com) using background adjustment, quantile normalization and median polish summarization.
|
| |
|
| Submission date |
Nov 16, 2010 |
| Last update date |
Sep 16, 2011 |
| Contact name |
Susan K. Murphy |
| Organization name |
Duke University Medical Center
|
| Department |
Obstetrics and Gynecology
|
| Street address |
B226 LSRC B Wing, Research Drive, Box 91012
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708 |
| Country |
USA |
| |
|
| Platform ID |
GPL3921 |
| Series (2) |
| GSE25428 |
Gene expression profiles of ovarian cancer cell lines in the presence and absence of a DNA methyltransferase inhibitor |
| GSE25429 |
Gene expression profiles of primary cultured ovarian cells and ovarian cancer cell lines in the presence and absence of a DNA methyltransferase inhibitor |
|
