|
| Status |
Public on Aug 02, 2012 |
| Title |
C2i-Myog + Dox replicate II |
| Sample type |
RNA |
| |
|
| Source name |
C2C12 modified line
|
| Organism |
Mus musculus |
| Characteristics |
cell line: C2i-Myog (a stable C2C12-derived cell line)
protocol: Differentiation with Dox treatment
|
| Treatment protocol |
Near confluence, myoblasts were induced to differentiate through serum withdrawl using a DMEM containing 1% horse serum, insulin (10 µg/mL), and transferrin (10 µg/mL) for 48 h. Where indicated, expression of exogenous Flag-tagged mouse Myog was induced at the time of differentiation through the addition of 0.2 µg/mL of Dox (Sigma). Inhibition of p38a/b MAPK signaling was established through the addition of 10 µM SB (Calbiochem) at the time of differentiation and maintained for 48 hrs. Cells were incubated Dox-treated (to control for effects of Dox – but also leads to increased Myog expression); SB treated (blocks p38 MAPK signaling); and SB and Dox treated (blocks p38 MAPK signaling will allowing expression of exogenous Flag-Myog).
|
| Growth protocol |
C2C12 derivative stable cell lines were maintained in proliferative conditions at a cell density of less than 70% confluency in DMEM containing 10% FCS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Whole transcript sense target labeling assay
|
| |
|
| Hybridization protocol |
Affymetrix standard
|
| Scan protocol |
Affymetrix standard
|
| Description |
Expresses p38 & Myog
Affy3303.CEL
|
| Data processing |
The data were analyzed with RMA as background correction, summarization & normalization method. RMA data was produced with the Expression Console software from Affymetrix.
|
| |
|
| Submission date |
Dec 01, 2010 |
| Last update date |
Aug 02, 2012 |
| Organization |
Ottawa Hospital Research Institute |
| Phone |
(613) 737-8899 -73255
|
| Department |
Cellular and Molecular Medicine
|
| Lab |
Ottawa Bioinformatics Core Facility
|
| Street address |
501 Smyth Rd.
|
| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1H 8L6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE25763 |
Comparative expression profiling identifies differential roles for Myogenin and p38α MAPK signaling in myogenesis |
|
