|
| Status |
Public on Jul 30, 2022 |
| Title |
input |
| Sample type |
SRA |
| |
|
| Source name |
Intestinal epithelium, P7
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Intestinal epithelium, P7
cell line: NA
cell type: Mixed primary intestinal epithelial
genotype: WT CD1
antibody: NA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Single cells were isolated from medial intestinal epithelium of CD1 mice at postnatal day 7. CUT&RUN Assay Kit from Cell Signaling Technologies was used to perform the CUT&RUN. The MinElute PRC Purification Kit from Quiagen was used to isolate the DNA.
Libraries were prepared as previously described (Meers et al., 2019; Skene and Henikoff, 2017).
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
The adapter were trimmed by trimgalore (v0.6.6)
The reads were aligned to mm10 genome by bowtie2 (v 2.4.2)
The algnment coverage were created by deeptools (v 3.5.1)
Supplementary files format and content: bigwig files with coverage normalized by CPM
Library strategy: Cut & Run
|
| |
|
| Submission date |
Jul 27, 2022 |
| Last update date |
Jul 31, 2022 |
| Contact name |
Anne (Maggie) M Bara |
| Organization name |
Duke University
|
| Department |
Cell Biology and Dermatology
|
| Lab |
Lechler
|
| Street address |
307 Research Dr.
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE209867 |
MafB binding in P7 intestinal epithelium |
|
| Relations |
| BioSample |
SAMN29993366 |
| SRA |
SRX16688463 |
