|
| Status |
Public on Dec 15, 2011 |
| Title |
Leucoraja erinacea pooled Stage 20-29 embryos |
| Sample type |
SRA |
| |
|
| Source name |
Stage 20-29 embryonic
|
| Organism |
Leucoraja erinacea |
| Characteristics |
developmental stage: Stage 20-29 embryos
lineage: elasmobranch
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Tri Reagent (Ambion) from pooled tissue samples and converted into a library of template molecules using the mRNA-Seq Sample Prep Kit (Illumina) according to manufacturer's protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
Six pooled stage 20-29 Leucoraja erinacea embryos
|
| Data processing |
Reads from each species exported by Illumina's pipeline software were trimmed and assembled using CLCBio Genome Workbench version 4 (http://www.clcbio.com).
contig_read_counts.csv file: RNAseq data formatted as number of reads that map to a particular transcriptome assembly contig #.
contigs.fa file: The transcriptome assembly contigs.
|
| |
|
| Submission date |
Dec 21, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Benjamin L King |
| E-mail(s) |
benjamin.l.king@maine.edu
|
| Phone |
207-581-2803
|
| Organization name |
University of Maine
|
| Department |
Molecular and Biomedical Sciences
|
| Street address |
5735 Hitchner Hall
|
| City |
Orono |
| State/province |
ME |
| ZIP/Postal code |
04469 |
| Country |
USA |
| |
|
| Platform ID |
GPL11347 |
| Series (1) |
| GSE26235 |
Late-stage embryonic gene expression in three cartilaginous fishes |
|
| Relations |
| SRA |
SRX036536 |
| BioSample |
SAMN00188953 |
