|
| Status |
Public on Jun 21, 2012 |
| Title |
Untreated Control 1 |
| Sample type |
RNA |
| |
|
| Source name |
Human epithelial pancreas
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Mia Paca-2
treatment: Untreated Control
|
| Treatment protocol |
Mia Paca-2 cells were treated in the presence and absence of IL-1β with the concentration of 2ng/ml for 36 hours
|
| Growth protocol |
Human epithelial pancreatic cell line Mia Paca-2 were cultured in DMEM high glucose supplemented with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
botin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Amplification kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol on WG-6_V3 chip
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Control Rep1
|
| Data processing |
The data were normalised using average normalisation with Illumina bead studio software v 3.3.7 and significant genes were with differential scores ≥±13.
|
| |
|
| Submission date |
Jan 19, 2011 |
| Last update date |
Jun 21, 2012 |
| Contact name |
Malabika Datta |
| E-mail(s) |
mdatta@igib.res.in
|
| Phone |
91 11 27667602
|
| Organization name |
Institute of Genomics and Integrative Biology
|
| Street address |
Mall Road
|
| City |
Delhi |
| ZIP/Postal code |
110 007 |
| Country |
India |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE26702 |
Genome wide analysis of pancreatic gene expression by Interleukin 1β in human epithelial pancreatic Mia Paca-2 cells. |
|
