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Sample GSM66246 Query DataSets for GSM66246
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae BY4743 YPD EM93 YPD Array_A
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae BY4743 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae BY4743 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae EM93 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae EM93 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -0.30 424 74 521 135 0
2 -0.24 1427 114 1690 192 0
3 -0.05 3400 51 3526 116 0
4 -0.48 217 78 302 159 0
5 0.76 13255 63 7847 125 0
6 0.20 2218 87 1926 171 0
7 0.34 722 83 572 160 0
8 -0.37 321 79 415 154 0
9 -0.24 616 55 730 116 0
10 -0.76 404 68 686 156 0
11 0.52 7649 68 5340 137 0
12 -0.44 709 113 959 222 0
13 -0.09 1750 75 1865 159 0
14 -0.10 742 87 798 184 0
15 -0.04 2209 82 2264 167 0
16 -0.27 292 39 353 120 0
17 -0.52 268 57 383 120 0
18 -0.11 1428 101 1536 177 0
19 -0.13 1090 54 1193 125 0
20 0.31 2376 83 1919 170 0

Total number of rows: 6218

Table truncated, full table size 174 Kbytes.




Supplementary data files not provided

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