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Sample GSM66248 Query DataSets for GSM66248
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae BY4743 YPD EM93 YPD Replicate1_Array_A
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae BY4743 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae BY4743 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae EM93 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae EM93 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -0.58 134 40 201 99 0
2 0.09 404 40 380 101 0
3 -0.09 1926 42 2057 108 0
4 -0.92 64 42 121 100 -50
5 0.61 4733 44 3101 102 0
6 0.25 1126 44 947 105 0
7 0.55 474 44 323 104 0
8 -0.06 268 46 280 101 0
9 -0.22 247 52 287 107 0
10 -0.55 151 53 221 99 0
11 0.12 1997 51 1832 94 0
12 -0.37 215 57 277 104 0
13 0.06 359 58 345 106 0
14 -0.24 218 56 258 102 0
15 0.40 942 63 716 105 0
16 -0.47 111 50 154 93 0
17 -1.58 54 41 162 105 0
18 -0.11 870 41 940 104 0
19 -0.12 364 42 396 105 0
20 -0.14 746 43 824 104 0

Total number of rows: 6218

Table truncated, full table size 166 Kbytes.




Supplementary data files not provided

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