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Sample GSM66249 Query DataSets for GSM66249
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae BY4743 YPD EM93 YPD Replicate1_Array_B
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae BY4743 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae BY4743 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae EM93 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae EM93 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -0.81 94 44 165 91 0
2 -0.02 354 37 359 86 0
3 -0.23 1722 41 2015 93 0
4 -0.58 181 41 270 92 0
5 0.45 4436 44 3245 94 0
6 0.09 946 44 887 93 0
7 0.51 362 44 255 93 0
8 -0.29 213 44 260 93 0
9 -0.38 190 52 247 93 0
10 -0.47 168 64 232 104 0
11 0.13 1840 49 1684 91 0
12 -0.19 233 59 265 101 0
13 -0.13 265 53 290 93 0
14 -0.14 231 55 255 97 0
15 0.25 766 47 645 87 0
16 -0.57 83 54 123 97 -50
17 -1.49 44 42 124 94 -50
18 -0.25 865 40 1026 89 0
19 -0.27 312 42 377 95 0
20 -0.35 692 42 881 90 0

Total number of rows: 6218

Table truncated, full table size 163 Kbytes.




Supplementary data files not provided

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