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Sample GSM66250 Query DataSets for GSM66250
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae BY4743 YPD EM93 YPD Replicate_2
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae BY4743 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae BY4743 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae EM93 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae EM93 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -1.09 41 35 87 75 -50
2 -0.15 169 38 187 79 0
3 -0.39 1227 40 1611 75 0
4 -0.45 205 41 280 81 0
5 0.44 3577 44 2628 81 0
6 -0.04 556 41 571 82 0
7 0.47 297 43 215 81 0
8 -0.43 143 44 193 82 0
9 -0.29 241 46 294 81 0
10 -0.64 139 46 217 80 0
11 -0.34 1924 44 2442 76 0
12 -0.55 197 42 288 77 0
13 -0.49 306 42 429 77 0
14 -0.71 158 42 259 76 0
15 -0.24 728 41 858 73 0
16 -0.71 96 42 157 78 0
17 -1.70 42 36 136 78 0
18 -0.25 553 39 659 80 0
19 -0.46 190 41 262 79 0
20 -0.36 417 40 535 80 0

Total number of rows: 6218

Table truncated, full table size 162 Kbytes.




Supplementary data files not provided

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