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Sample GSM66253 Query DataSets for GSM66253
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae EM93 NS24 Sg60 NS24 Array_A
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae EM93 in NS24
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae EM93 in NS24
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sg60 in NS24
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sg60 in NS24
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -1.35 127 32 324 52 0
2 -1.56 91 32 269 71 0
3 -2.29 362 32 1769 62 0
4 -1.62 174 36 536 77 0
5 1.24 9302 31 3935 56 0
6 -1.35 173 32 440 68 0
7 -1.08 247 31 522 61 0
8 -1.73 114 32 377 70 0
9 -2.05 129 32 533 64 0
10 -2.06 90 32 375 66 0
11 -0.57 566 32 838 62 0
12 -1.75 323 33 1087 72 0
13 -1.56 356 33 1047 71 0
14 -1.21 221 33 513 74 0
15 -1.46 611 32 1678 65 0
16 -1.55 85 31 249 66 0
17 -1.19 208 31 474 62 0
18 -1.82 262 33 924 77 0
19 -1.34 269 33 683 66 0
20 -1.74 343 31 1144 70 0

Total number of rows: 6218

Table truncated, full table size 164 Kbytes.




Supplementary data files not provided

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