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Sample GSM66255 Query DataSets for GSM66255
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae EM93 NS4 EM93 YPD Array_A
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae EM93 in NS4
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae EM93 in NS4
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae EM93 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae EM93 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 2.82 17179 57 2427 91 0
2 -1.03 945 52 1935 90 0
3 -0.77 687 56 1173 93 0
4 -0.59 352 49 529 86 0
5 -1.02 3931 60 7994 110 0
6 -0.77 828 62 1409 102 0
7 -0.79 5422 65 9379 116 0
8 -0.87 1633 57 2989 105 0
9 -0.07 2104 72 2214 123 0
10 0.66 4914 72 3110 130 0
11 -1.02 773 62 1567 110 0
12 -0.68 390 73 626 124 0
13 -0.25 1769 58 2097 113 0
14 1.87 6696 71 1838 122 0
15 0.20 5408 59 4704 107 0
16 0.86 5382 55 2975 107 0
17 -0.78 795 54 1365 91 0
18 0.42 19905 55 14892 96 0
19 -0.60 1080 54 1632 94 0
20 -0.73 1383 51 2290 91 0

Total number of rows: 6218

Table truncated, full table size 174 Kbytes.




Supplementary data files not provided

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