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Sample GSM66256 Query DataSets for GSM66256
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae EM93 NS4 EM93 YPD Array_B
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae EM93 in NS4
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae EM93 in NS4
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae EM93 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae EM93 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 2.96 14277 49 1831 83 0
2 -0.73 707 52 1175 80 0
3 -1.09 328 51 699 86 0
4 -0.37 428 48 554 82 0
5 -1.07 1907 53 3994 90 0
6 -0.41 895 57 1188 100 0
7 -0.87 3409 56 6225 100 0
8 -0.41 2923 55 3886 94 0
9 -0.93 400 68 764 116 0
10 0.69 5120 64 3165 110 0
11 -1.09 519 61 1108 105 0
12 -0.68 404 62 646 111 0
13 -0.37 1638 55 2115 94 0
14 2.00 6226 58 1561 107 0
15 0.18 5357 59 4729 102 0
16 0.47 3796 53 2732 98 0
17 -0.76 455 51 772 83 0
18 0.02 12448 49 12307 82 0
19 -0.67 593 52 944 90 0
20 -0.05 4031 56 4171 92 0

Total number of rows: 6218

Table truncated, full table size 173 Kbytes.




Supplementary data files not provided

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