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Sample GSM66260 Query DataSets for GSM66260
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae EM93 YPD EM93 NS24 Array_B
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae EM93 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae EM93 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae EM93 in NS24
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae EM93 in NS24
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -1.26 66 45 158 113 0
2 -1.13 77 57 168 122 0
3 1.73 1069 47 322 125 0
4 -0.85 104 51 188 120 0
5 0.98 486 48 247 123 0
6 -0.70 117 52 190 121 0
7 -1.78 55 51 189 123 0
8 -1.35 70 49 178 117 0
9 -1.95 54 42 208 115 0
10 -1.63 67 59 208 144 0
11 -0.23 351 44 411 122 0
12 -1.53 69 57 199 132 0
13 -1.29 70 47 171 128 0
14 -1.47 73 59 202 124 0
15 -0.56 158 47 233 128 0
16 -0.65 145 52 227 119 0
17 -1.75 52 46 175 117 0
18 -1.04 87 58 179 124 0
19 -1.74 60 44 200 119 0
20 -1.09 86 64 183 123 0

Total number of rows: 6218

Table truncated, full table size 164 Kbytes.




Supplementary data files not provided

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