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Sample GSM66264 Query DataSets for GSM66264
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Ds288c NS24 Sgu52 NS24 Array_A
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Ds288c in NS24
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Ds288c in NS24
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sgu52 in NS24
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu52 in NS24
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -1.39 102 66 267 144 0
2 -1.37 425 79 1095 229 0
3 -0.43 1042 79 1400 185 0
4 -1.50 440 70 1247 176 0
5 0.06 10962 78 10488 184 0
6 0.36 1408 91 1096 255 0
7 -1.73 371 88 1231 228 0
8 -2.12 379 81 1646 253 0
9 -1.62 200 64 615 208 0
10 -1.25 496 73 1179 223 0
11 2.09 2537 62 595 176 0
12 -1.39 1028 81 2689 259 0
13 -1.09 647 48 1377 127 0
14 -1.66 427 74 1345 224 0
15 -2.06 1039 50 4326 121 0
16 -1.78 632 69 2169 197 0
17 -1.06 164 64 343 138 0
18 -1.15 806 78 1787 232 0
19 -0.89 1019 75 1886 191 0
20 -0.65 1376 74 2153 182 0

Total number of rows: 6218

Table truncated, full table size 176 Kbytes.




Supplementary data files not provided

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