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Sample GSM66265 Query DataSets for GSM66265
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Ds288c NS24 Sgu52 NS24 Array_B
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Ds288c in NS24
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Ds288c in NS24
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sgu52 in NS24
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu52 in NS24
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -1.18 62 54 140 129 -50
2 -1.58 372 62 1112 189 0
3 -0.72 1477 51 2428 118 0
4 -1.54 345 70 1004 193 0
5 -0.96 3828 58 7431 139 0
6 0.03 1131 70 1110 209 0
7 -2.20 135 51 620 131 0
8 -2.23 362 63 1700 183 0
9 -1.36 285 57 734 127 0
10 -1.58 500 69 1495 201 0
11 2.14 1985 76 449 183 -50
12 -1.63 787 66 2431 197 0
13 -1.21 922 139 2128 357 0
14 -1.69 408 58 1314 152 0
15 -1.93 1099 78 4202 208 0
16 -2.25 175 63 831 194 0
17 -1.37 163 56 420 141 0
18 -0.98 680 63 1345 192 0
19 -1.21 413 50 958 116 0
20 -0.92 1026 67 1945 206 0

Total number of rows: 6218

Table truncated, full table size 175 Kbytes.




Supplementary data files not provided

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