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Sample GSM66266 Query DataSets for GSM66266
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Ds288c NS4 Ds288c YPD Array_A
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Ds288c in NS4
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Ds288c in NS4
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Ds288c in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Ds288c in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 1.05 2482 31 1201 49 0
2 -2.02 393 31 1589 49 0
3 -2.24 83 34 392 52 0
4 -1.17 84 33 189 54 0
5 -2.66 425 36 2691 56 0
6 -1.21 130 36 301 59 0
7 -2.07 658 37 2756 58 0
8 -1.19 486 36 1109 59 0
9 -0.44 1331 42 1807 69 0
10 0.43 1002 42 745 68 0
11 -4.41 288 43 6110 69 0
12 -0.98 125 41 246 67 0
13 -0.75 1190 40 2000 67 0
14 0.48 468 38 336 65 0
15 -0.28 1877 38 2281 64 0
16 -0.31 691 36 855 65 0
17 -0.68 259 31 414 51 0
18 -0.98 2124 31 4195 50 0
19 -1.17 230 34 516 52 0
20 -1.35 343 35 873 55 0

Total number of rows: 6218

Table truncated, full table size 163 Kbytes.




Supplementary data files not provided

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