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Sample GSM66267 Query DataSets for GSM66267
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Ds288c NS4 Ds288c YPD Array_B
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Ds288c in NS4
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Ds288c in NS4
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Ds288c in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Ds288c in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 2.04 3947 29 960 49 0
2 -1.30 247 29 607 49 0
3 -1.92 64 33 243 53 0
4 -1.41 65 31 173 52 0
5 -2.84 335 33 2393 57 0
6 -1.84 84 34 300 54 0
7 -2.05 589 34 2443 58 0
8 -1.64 187 35 583 58 0
9 -0.64 641 41 999 66 0
10 -0.23 727 41 850 66 0
11 -4.45 174 40 3802 63 0
12 -0.99 93 38 185 65 0
13 -0.63 488 35 753 64 0
14 0.80 540 35 310 63 0
15 -0.24 1112 36 1315 62 0
16 -0.27 692 37 834 64 0
17 -0.85 174 29 313 51 0
18 -1.13 1399 29 3059 51 0
19 -0.99 202 32 400 53 0
20 -1.04 61 32 125 54 0

Total number of rows: 6218

Table truncated, full table size 162 Kbytes.




Supplementary data files not provided

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