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Sample GSM66276 Query DataSets for GSM66276
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Ds288c NS4 Ds288c SWM
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Ds288c in NS4
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Ds288c in NS4
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Ds288c in SWM
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Ds288c in SWM
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 0.56 139 99 94 47 0
2 0.50 189 101 134 44 0
3 -1.04 866 103 1778 48 0
4 1.08 116 100 55 45 -50
5 -0.21 3390 118 3927 54 0
6 -0.37 1044 106 1352 47 0
7 0.96 419 117 215 52 0
8 0.27 346 102 287 48 0
9 -0.36 364 101 467 51 0
10 -0.02 328 107 333 52 0
11 1.25 5752 105 2418 51 0
12 0.65 994 97 633 48 0
13 0.78 1354 108 787 54 0
14 0.43 433 104 322 50 0
15 -0.35 1435 98 1827 49 0
16 0.73 161 100 97 47 0
17 1.18 111 102 49 47 -50
18 -0.76 562 104 953 44 0
19 0.49 432 110 307 50 0
20 0.59 1091 103 724 45 0

Total number of rows: 6218

Table truncated, full table size 165 Kbytes.




Supplementary data files not provided

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