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Sample GSM66278 Query DataSets for GSM66278
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Ds288c YPD BY4743 YPD Array_B
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Ds288c in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Ds288c in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae BY4743 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae BY4743 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -0.04 759 54 780 87 0
2 0.15 2362 83 2135 113 0
3 0.21 13934 69 12059 98 0
4 0.16 2644 145 2372 179 0
5 0.66 27721 97 17572 125 0
6 0.21 8466 159 7324 199 0
7 0.24 3482 101 2951 123 0
8 0.12 1939 162 1781 205 0
9 -0.16 1690 127 1893 156 0
10 -0.08 1126 96 1189 129 0
11 0.92 21157 131 11183 155 0
12 -0.19 1737 89 1983 123 0
13 -0.02 3468 139 3527 160 0
14 0.17 2676 115 2373 150 0
15 0.16 9297 88 8337 105 0
16 -0.31 159 133 197 167 -50
17 -0.61 106 60 162 92 0
18 -0.01 2433 86 2447 117 0
19 -0.09 1807 77 1925 106 0
20 -0.05 3654 155 3771 192 0

Total number of rows: 6218

Table truncated, full table size 182 Kbytes.




Supplementary data files not provided

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