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Sample GSM66281 Query DataSets for GSM66281
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sg60 NS4 Sg60 YPD Array_A
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sg60 in NS4
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sg60 in NS4
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sg60 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sg60 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 0.86 759 78 419 122 0
2 0.75 452 107 269 143 0
3 0.56 8142 83 5525 137 0
4 0.29 512 93 420 140 0
5 1.20 16739 78 7264 125 0
6 0.62 2891 112 1882 150 0
7 0.97 970 85 496 128 0
8 0.78 1163 124 675 160 0
9 1.01 1785 214 888 234 0
10 0.44 1128 269 829 262 0
11 0.89 10303 98 5551 169 0
12 0.61 1486 214 973 218 0
13 0.25 1977 104 1668 156 0
14 0.37 870 160 671 190 0
15 0.95 3927 152 2026 176 0
16 0.49 851 136 604 181 0
17 0.57 641 89 431 133 0
18 0.69 1992 106 1234 145 0
19 0.34 1411 85 1116 136 0
20 0.99 3114 106 1572 148 0

Total number of rows: 6218

Table truncated, full table size 173 Kbytes.




Supplementary data files not provided

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