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Sample GSM66282 Query DataSets for GSM66282
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sg60 NS4 Sg60 YPD Array_B
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sg60 in NS4
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sg60 in NS4
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sg60 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sg60 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -1.34 104 46 263 185 0
2 0.90 1937 220 1036 254 0
3 0.30 7934 120 6430 204 0
4 0.25 409 134 345 201 0
5 0.99 16080 183 8120 255 0
6 0.50 2467 97 1739 184 0
7 0.71 1081 149 659 206 0
8 0.36 803 85 625 171 0
9 -1.23 194 64 455 235 0
10 0.50 1235 460 875 421 0
11 0.59 9771 305 6487 321 0
12 0.58 1863 380 1247 371 0
13 0.10 2167 271 2017 310 0
14 0.25 988 263 831 309 0
15 0.77 4350 412 2545 390 0
16 0.47 2320 206 1672 263 0
17 -1.16 137 48 306 179 0
18 0.46 2140 196 1552 232 0
19 0.14 1584 135 1439 220 0
20 0.74 2838 153 1705 229 0

Total number of rows: 6218

Table truncated, full table size 176 Kbytes.




Supplementary data files not provided

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