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Sample GSM66283 Query DataSets for GSM66283
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sg60 SWM Sg60 NS24 Array_A
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sg60 in SWM
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sg60 in SWM
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sg60 in NS24
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sg60 in NS24
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -1.32 176 52 438 52 0
2 -2.96 161 55 1254 70 0
3 -0.59 4037 52 6092 59 0
4 -1.71 172 54 562 70 0
5 -2.54 1833 51 10637 63 0
6 0.24 773 53 656 70 0
7 -2.13 218 50 951 60 0
8 -1.99 169 51 670 67 0
9 -1.76 165 55 560 59 0
10 -1.59 201 62 607 74 0
11 -0.29 830 58 1015 68 0
12 -2.21 151 60 697 73 0
13 -1.91 246 49 923 60 0
14 -1.33 209 63 525 73 0
15 -2.09 402 52 1710 62 0
16 -1.82 232 61 817 70 0
17 0.22 137 50 118 53 0
18 -2.20 300 57 1379 71 0
19 -1.56 215 51 634 60 0
20 -1.93 257 55 979 72 0

Total number of rows: 6218

Table truncated, full table size 166 Kbytes.




Supplementary data files not provided

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