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Sample GSM66287 Query DataSets for GSM66287
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sg60 YPD Sg60 NS24 Array_A
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sg60 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sg60 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sg60 in NS24
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sg60 in NS24
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -1.22 428 74 1000 288 0
2 -0.52 795 81 1141 310 0
3 -2.05 9334 78 38536 310 0
4 -1.69 594 75 1916 295 0
5 -1.85 9062 92 32583 358 0
6 -2.69 456 75 2938 318 0
7 -0.29 1150 94 1402 326 0
8 -1.52 293 74 842 319 0
9 -1.69 402 78 1296 290 0
10 -0.90 752 84 1399 287 0
11 -3.61 759 71 9294 277 0
12 -2.01 158 69 636 250 0
13 -1.07 899 76 1887 282 0
14 -1.23 873 81 2045 290 0
15 -1.13 1665 79 3647 282 0
16 -0.83 1893 83 3374 296 0
17 -2.46 114 76 626 295 0
18 -1.31 970 83 2401 316 0
19 -1.19 638 77 1458 304 0
20 -1.00 380 78 758 315 0

Total number of rows: 6218

Table truncated, full table size 177 Kbytes.




Supplementary data files not provided

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