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Sample GSM66289 Query DataSets for GSM66289
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sg60 YPD Sg60 NS24 Replicate1_Array_A
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sg60 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sg60 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sg60 in NS24
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sg60 in NS24
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -1.06 280 42 583 92 0
2 -1.34 273 48 691 95 0
3 1.35 14073 45 5515 91 0
4 -0.57 535 46 796 92 0
5 0.69 8182 46 5058 94 0
6 1.47 1395 44 502 88 0
7 -1.81 203 44 711 94 0
8 -0.85 227 42 409 90 0
9 -0.48 279 43 389 91 0
10 -0.72 260 50 429 102 0
11 1.82 2139 46 605 96 0
12 -0.93 188 48 357 101 0
13 -0.67 335 42 533 86 0
14 -0.56 277 44 409 97 0
15 0.02 985 41 973 88 0
16 -0.41 742 42 986 94 0
17 -1.29 97 42 237 94 0
18 0.09 778 47 733 95 0
19 -0.81 289 44 505 91 0
20 -0.51 487 45 692 91 0

Total number of rows: 6218

Table truncated, full table size 164 Kbytes.




Supplementary data files not provided

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