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Sample GSM66293 Query DataSets for GSM66293
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sg60 YPD BY4743 YPD Array_A
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sg60 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sg60 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae BY4743 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae BY4743 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -0.53 79 68 114 93 -50
2 -0.99 1656 111 3279 134 0
3 -0.29 9098 58 11139 79 0
4 -0.10 95 109 102 122 -50
5 -1.44 5960 65 16211 97 0
6 -0.34 7112 95 9017 107 0
7 -1.41 1591 60 4235 91 0
8 -0.62 1660 91 2556 102 0
9 -0.70 1928 53 3141 62 0
10 -0.93 959 85 1833 86 0
11 -0.69 9030 73 14616 104 0
12 -0.40 2273 78 3009 77 0
13 -0.80 3121 58 5438 70 0
14 -0.58 1839 86 2740 84 0
15 -0.58 7336 72 10941 96 0
16 -0.90 293 95 548 83 0
17 -0.38 59 63 77 87 -50
18 -0.49 3986 101 5591 120 0
19 -0.21 2204 74 2551 88 0
20 -0.24 113 106 133 126 -50

Total number of rows: 6218

Table truncated, full table size 175 Kbytes.




Supplementary data files not provided

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