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Sample GSM66294 Query DataSets for GSM66294
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sg60 YPD BY4743 YPD Array_B
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sg60 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sg60 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae BY4743 in YPD
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae BY4743 in YPD
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -0.43 788 52 1059 54 0
2 -0.78 1600 52 2754 61 0
3 -0.21 7876 68 9091 70 0
4 -0.17 56 56 63 65 -50
5 -1.12 10800 67 23411 78 0
6 -0.20 7400 67 8479 73 0
7 -1.03 2274 63 4657 79 0
8 -0.38 2050 69 2665 78 0
9 -0.93 1562 66 2984 79 0
10 -0.93 1253 66 2392 78 0
11 -0.39 11354 79 14911 90 0
12 -0.52 2618 75 3743 86 0
13 -0.66 3288 67 5179 75 0
14 -0.51 2194 74 3130 85 0
15 -0.39 7643 67 10004 78 0
16 -0.77 215 68 367 76 0
17 -0.02 61 53 62 57 -50
18 -0.69 2839 55 4568 61 0
19 0.11 79 62 73 69 -50
20 -0.21 65 58 75 65 -50

Total number of rows: 6218

Table truncated, full table size 174 Kbytes.




Supplementary data files not provided

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