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Sample GSM66295 Query DataSets for GSM66295
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sgu407 NS24 Sgu421 NS24 Array_A
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sgu407 in NS24
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu407 in NS24
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae Sgu421 in NS24
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu421 in NS24
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) duccio.cavalieri@unifi.it
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -0.21 154 45 178 81 0
2 -0.68 196 65 315 126 0
3 -0.96 645 46 1259 89 0
4 -0.38 501 70 650 127 0
5 0.23 5288 45 4495 87 0
6 -0.02 682 93 690 163 0
7 -0.89 148 42 275 79 0
8 -0.04 669 94 688 168 0
9 -0.59 191 48 288 86 0
10 -0.51 509 97 723 194 0
11 -1.02 1468 50 2978 86 0
12 -0.19 905 100 1029 187 0
13 -0.05 671 47 693 85 0
14 -0.02 485 125 493 221 0
15 0.06 1076 53 1035 97 0
16 -0.37 1033 138 1338 236 0
17 -0.48 239 46 334 81 0
18 -0.95 478 65 922 122 0
19 -0.05 674 47 697 89 0
20 -0.51 810 70 1150 126 0

Total number of rows: 6218

Table truncated, full table size 168 Kbytes.




Supplementary data files not provided

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